ANALISIS JUMLAH KROMOSOM PADA TRIPLOIDISASI IKAN MAS (Cyprinus carpio Linn) RAS PUNTEN DENGAN LAMA PERENDAMAN KEJUT SUHU PANAS YANG BERBEDA

Abstract

Goldfish (Cyprinus carpio Linn) punten breeds is a type of freshwater fish that has great
potential in terms of quality and quantity. Seed production from generation to generation has
decreased quality. An alternative effort was made to maintain the seed quality of genetic
engineering with triploidization process using heat shock treatment. The heat shock can prevent
polar body II from splitting on eggs during cleavage of meiosis II level resulting in 3N
chromosome or sterile fish. The purpose of this research is to analyze chromosome number in
triploidization process of punten breeds fish and to analyze the effect of temperature shock
duration on hatching rate of goldfish (Cyprinus carpio Linn) Punten Breeds. This research was
descriptive research with 2 treatments and 3 replications in each treatment that was heat shock
treatment 40 ºC at minute 2 after fertilization with difference of heat shock duration 1,5 minutes
(treatment A) and shock time temperature 2 minutes (treatment B). The parameters observed
were triploid success, hatching rate, survival rate, and growth of of goldfish (Cyprinus carpio
Linn) Punten Breeds, analyzed by T-test with 95% confidence interval. The result of heat shock
duration of 1.5 minutes showed the percentage of triploid fish 53,57% and shock time
temperature 2 minute 43,64%. The result of T-test of both treatments had no significant effect on
all observation parameters but had varied relationship that was enough and inversely related to
all observation parameters