Antioxidant Activity of Ethanol Extract 96% Gelidium zollingeri with DPPH Method (2,2-diphenyl-1-picryllhydrazyl)

Abstract

Gelidium zollingeri is a Rhodophyta seaweed that has compounds such as Mycosporine-like amino acids (MAAs) are believed to have a role as antioxidants. Antioxidants are chemical compounds that can inhibit or neutralize free radicals by complementing the electrons possessed by free radicals, thereby breaking the chain reaction of free radical formation. This research was conducted to determine the antioxidant activity of 96% ethanol extract of the red seaweed Gelidium zollingeri. Antioxidant activity testing was carried out using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The 96% ethanol extract of the red seaweed Gelidium zollingeri was tested for its antioxidant activity to obtain absorbance using a UV-Vis spectrophotometer at a wavelength of 517 nm. The absorbance results were calculated to obtain the IC50 value. The results of the antioxidant activity test of the 96% ethanol extract of the red seaweed Gelidium zollingeri using a UV-Vis spectrophotometer obtained an IC50 value of 39.2598 ppm, which means that the extract is included in the very strong antioxidant category, so that the red seaweed plant Gelidium zollingeri has the potential as an antioxidant.