ISOLASI DAN IDENTIFIKASI BAKTERI PENDEGRADASI SELULOSA ASAL EKOSISTEM MANGROVE TUKAK SADAI, BANGKA SELATAN

Abstract

Perikanan dan Kelautan, Universitas Brawijaya
3Dosen Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya.
ABSTRACT
Agricultural waste has problems on fiber and cellulose digestibility in its utilization for
aquaculture. The ability of plant cellulose degrading bacteria to become a source of
energy can increase the digestibility of feed by fish. The purpose of this study was to
identify the cellulolytic bacteria from the mangrove ecosystem, Tukak Sadai District,
South Bangka Regency. This study was conducted in March until August 2017.
Samples were taken from litter, mud and weathered wood and isolated using
Carboxymetyl Cellulosa (CMC) 1% media and found 22 bacterial isolates. Gram
staining results showed that 3 isolates (TSS 2, TSL 6 and TSK 4) were classified as
gram positive and 19 other isolates were gram negative. Cellulolytic test results showed
6 isolates had the ability to degrade cellulase, namely 3 isolates from mangrove mud
samples (TSS 1, TSL 7, TSL 1), 2 isolates from leaf litter (TSS 4, TSL 2) and 1 isolate
from weathered wood (TSK 5) . Sequencing of 16S rRNA gene DNA showed proximity
to Pseudomonas aeruginosa in TSL 7 and TSS isolates 4