PEMANFAATAN SERBUK DAUN SIRIH (Piper betle) UNTUK MENINGKATKAN KESEHATAN IKAN NILA (Oreochromis niloticus)

Abstract

This research was conducted to determine the effectiveness of betel leaf powder mixed in
the feed (pellet) to improve the health status of tilapia by measuring the value of erythrocytes and
leukocytes. Measurements of erythrocyte values and blood leucocytes of tilapia were performed
after the test fish were fed with betel leaf powder (0.3 g / 100 g feed, 0.6 g / 100 g feed, 0.9 g /
100 g feed) for 6week maintenance period. While the control fish during the maintenance period
was fed pellet feed which was not mixed with betel leaf powder. The erythrocyte and leukocyte
values of tilapia were measured weekly by taking blood in the caudal vein section (tail) of the
test fish. Blood collection was performed using a sterile syringe size of 26 g, which was first
wetted EDTA (Ethylene diamine tetra acetic acid) As an anticoagulant of blood cells as
anticoagulants. Furthermore, the blood has been collected in Eppendorf tube which has also been
wetted EDTA (Ethylene diamine tetra acetic acid), then prepared by adding hayem and turk
solution and then measured the erythrocyte value and the leukocyte count with Haemometer. The
best measurements result was at the dose of 0.3 g / 100 g of feed which showed that the amount
of erythrocyte of tilapia increased from 1,145,000 cells / mm-3 (week I) to 1,986,667 cells / mm-3
(week IV) the number of leukocytes increased from 21,976 cells / mm-3 (week I) to 28,167 cells /
mm-3 (week IV). From the results of this study showed that the addition of betel leaf powder in
the feed can improve the health status of tilapia fish.